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Life after beanbag genetics: theoretical and empirical studies on epistasis and penetrance

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dc.contributor.advisor True, John R. en_US
dc.contributor.author Lachance, Joseph en_US
dc.contributor.other Department of Genetics en_US
dc.date.accessioned 2012-05-15T18:04:45Z
dc.date.accessioned 2015-04-24T14:45:00Z
dc.date.available 2012-05-15T18:04:45Z
dc.date.available 2015-04-24T14:45:00Z
dc.date.issued 2010-12-01 en_US
dc.identifier Lachance_grad.sunysb_0771E_10319.pdf en_US
dc.identifier.uri http://hdl.handle.net/1951/55514 en_US
dc.identifier.uri http://hdl.handle.net/11401/70889 en_US
dc.description.abstract Population genetics reduces the description of a population to a set of genotype frequencies. Because of this, it has been called"beanbag genetics.&quot. However, greater complexity and biological realism can be incorporated into this framework. Two examples of this include interactions between different genes (epistasis) and individuals with the same genotype that have different phenotypes (incomplete penetrance). During the course of my Ph.D. work, both of these topics were investigated using theoretical population genetics and Drosophila genetics. One unifying theme was that genes do not exist in isolation: they occur within genetic and environmental contexts. In turn, these contexts were found to affect the evolution of populations.The above considerations were addressed via four interlocking studies. First, we studied epistatic interactions between naturally segregating chromosomes in D. melanogaster. We placed a number of X chromosomes into multiple genetic backgrounds, finding that many X-autosome combinations were lethal or sterile. X-autosome incompatibilities also exhibited sex-specific and geographic patterns. Second, population genetics theory was extended to encompass X-autosome interactions. Allele frequency trajectories were calculated and found to be consistent with relatively high levels of segregating variation. This also led to testable predictions about the early stages of Haldane's rule. Third, we studied the incomplete penetrance of a naturally occurring mutation affecting wing development in D. melanogaster. This mutation was found to be allelic with the vesiculated gene. Subsequent tests examined how genetic background, temperature, sex, and maternal effects affected the presence and severity of wing vesiculation. Fourth, the theoretical population genetics of incomplete penetrance was developed. Mean fitness accurately predicted the evolutionary trajectories of beneficial alleles. However, fitness variance and maternal effects greatly affected the evolution of incompletely penetrant alleles that were neutral on average. Together, these four studies illustrate the complexity of natural genetic variation and suggest how it might have evolved. en_US
dc.description.sponsorship This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. en_US
dc.format Monograph en_US
dc.format.medium Electronic Resource en_US
dc.language.iso en_US en_US
dc.publisher The Graduate School, Stony Brook University: Stony Brook, NY. en_US
dc.subject.lcsh Genetics -- Mathematics en_US
dc.subject.other Drosophila, epistasis, penetrance, theoretical population genetics en_US
dc.title Life after beanbag genetics: theoretical and empirical studies on epistasis and penetrance en_US
dc.type Dissertation en_US
dc.mimetype Application/PDF en_US
dc.contributor.committeemember Walter F. Eanes en_US
dc.contributor.committeemember Daniel E. Dykhuizen en_US
dc.contributor.committeemember Joshua Rest en_US
dc.contributor.committeemember Stephen B. Munch en_US

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