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Heat shock protein, Hsp90 plays a role in MT1-MMP's ability to activate substrate proteins

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dc.contributor.advisor Cao, Jian en_US
dc.contributor.author Buck, Theresa en_US
dc.contributor.other Department of Biochemistry and Cell Biology en_US
dc.date.accessioned 2013-05-22T17:34:14Z
dc.date.accessioned 2015-04-24T14:45:35Z
dc.date.available 2013-05-22T17:34:14Z
dc.date.available 2015-04-24T14:45:35Z
dc.date.issued 2011-12-01 en_US
dc.identifier Buck_grad.sunysb_0771M_10741 en_US
dc.identifier.uri http://hdl.handle.net/1951/59591 en_US
dc.identifier.uri http://hdl.handle.net/11401/71017 en_US
dc.description 31 pg. en_US
dc.description.abstract Membrane type 1 matrix metalloproteinase (MT1-MMP) plays a major role in cancer invasion and metastasis. The mechanism by which MT1-MMP is trafficked to the plasma membrane where it functions to proteolytically activate its substrate proteins is not fully understood. The gene of heat shock protein (Hsp90alpha) was identified as being upregulated in HT1080 cells treated with concanavalin A (conA), a lectin that promotes proMMP-2 activation and cell migration in HT1080 cells compared to untreated HT1080 cells. To determine the mechanism underlying MT1-MMP substrate degradation and cell migration, biological and biochemical approaches were carried out. Zymography was preformed to examine the affect of the presence of both Hsp90alpha; and MT1-MMP, on the gelatinase activity of matrix metalloproteinase (MMP-2). The zympograph showed that when both Hsp90alpha and MT1-MMP were both present, there was an increase in the active form of MMP-2, a protein that is proteolytically activated by MT1-MMP at the plasma membrane. shRNA knockdown studies of Hsp90alpha were also done to further establish a role for Hsp90alpha in effecting the ability of MT1-MMP to activate its target proteins at the plasma membrane. Together these studies indicate that Hsp90alpha plays a role in the ability of MT1-MMP to activate substrate proteins at the plasma membrane. en_US
dc.description.sponsorship This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. en_US
dc.format Monograph en_US
dc.format.medium Electronic Resource en_US
dc.language.iso en_US en_US
dc.publisher The Graduate School, Stony Brook University: Stony Brook, NY. en_US
dc.subject.lcsh Biology en_US
dc.title Heat shock protein, Hsp90 plays a role in MT1-MMP's ability to activate substrate proteins en_US
dc.type Thesis en_US
dc.mimetype Application/PDF en_US
dc.contributor.committeemember Dean, Neta. en_US

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