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Human Enteric Viruses in Recreational Coastal Waters

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dc.contributor.advisor Aller, Josephine Y., Kemp, Paul en_US
dc.contributor.author Wochinger, Alexandra Valdes en_US
dc.contributor.other Department of Marine and Atmospheric Science en_US
dc.date.accessioned 2013-05-22T17:35:45Z
dc.date.accessioned 2015-04-24T14:47:35Z
dc.date.available 2013-05-22T17:35:45Z
dc.date.available 2015-04-24T14:47:35Z
dc.date.issued 2011-12-01 en_US
dc.identifier Valdes_grad.sunysb_0771E_10715 en_US
dc.identifier.uri http://hdl.handle.net/1951/59899 en_US
dc.identifier.uri http://hdl.handle.net/11401/71447 en_US
dc.description 109 pg. en_US
dc.description.abstract Human enteric viruses (HEV) are recognized by the CDC as common etiological agents for waterborne outbreaks and they have been detected in recreational waters in the US and around the world. Current concentration and detection methods present challenges to study of HEV in coastal waters and have prevented the routine monitoring of HEV in recreational or shellfish harvesting waters. In this dissertation, various methods of concentrating viruses from water were investigated. A two-step viral concentration method that included viral adsorption-elution and ultrafiltration was found to be effective for the concentration of total viruses and improved detection limits for HEV in coastal waters. This method was then applied in a time series study of human viral contamination to coastal recreational waters. RT-PCR was used to screen for three types of HEV: enteroviruses (EV), hepatitis A viruses (HAV) and noroviurses (NoV) in viral concentrates from surface waters of Port Jefferson Harbor (PJH) NY, which receives point and nonpoint sources of human waste. No HAV or NoV were detected in any samples, but EV was detected after precipitation events. Cloning and sequencing of the EV-positive samples revealed that the amplicons derived from strains of poliovirus. The results suggest that storm water runoff, which is discharged directly into the harbor, results in human viral contamination. Future studies of HEV sources and occurrence in the environment will help reduce public health risk to human viral pollution and transmission into coastal recreational waters, particularly after precipitation events. en_US
dc.description.sponsorship This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. en_US
dc.format Monograph en_US
dc.format.medium Electronic Resource en_US
dc.language.iso en_US en_US
dc.publisher The Graduate School, Stony Brook University: Stony Brook, NY. en_US
dc.subject.lcsh Biological oceanography--Public health en_US
dc.title Human Enteric Viruses in Recreational Coastal Waters en_US
dc.type Dissertation en_US
dc.mimetype Application/PDF en_US
dc.contributor.committeemember Taylor, Gordon T.Chistoserdov, Andrei en_US
dc.contributor.committeemember Steward, Grieg. en_US

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