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Water and Ion Transport in Lacrimal Gland: Models and Experiment

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dc.contributor.advisor Smolka, Scott A. en_US
dc.contributor.advisor Grosu, Radu en_US
dc.contributor.author Huang, Wei Chieh en_US
dc.contributor.other Department of Physiology and Biophysics en_US
dc.date.accessioned 2012-05-15T18:04:09Z
dc.date.accessioned 2015-04-24T14:52:34Z
dc.date.available 2012-05-15T18:04:09Z
dc.date.available 2015-04-24T14:52:34Z
dc.date.issued 2010-08-01 en_US
dc.identifier Huang_grad.sunysb_0771E_10263.pdf en_US
dc.identifier.uri http://hdl.handle.net/1951/55474 en_US
dc.identifier.uri http://hdl.handle.net/11401/72543 en_US
dc.description.abstract Tear secretion is a complicated process that involves two different cell types. The lacrimal acinar cell is thought to secrete a hypertonic primary fluid with a high K<super>+</super> concentration. This fluid is then modified by the ductal epithelia and transported to the avascular ocular surface. It is known that both sympathetic and parasympathetic input trigger protein release, but the role of adrenergic stimulation on water secretion in lacrimal gland remains unclear. Therefore, the first goal of this dissertation is to examine the influence of&#913;- and&#914;-adrenergic activation on tear fluid production.Tear secretion requires coordination of different membrane channels and transporters. Walcott et al. showed the importance of basolateral Na<super>+</super>-K<super>+</super>-2Cl<super>-</super>-cotransporter (NKCC1) on secretion rate. The second goal is to develop an acinar cell mathematical model with cell volume regulation. The model is used to examine whether NKCC1 alone is sufficient to generate hypertonic tear fluid with high KCl content when the cell is stimulated. Subsequently the model was expanded with acid-base regulatory transporters to investigate the interaction between volume- and pH-regulation systems. This effort led to the discovery that the sodium-bicarbonate transporter is expressed in lacrimal gland and participates in tear production.Lastly, since acinar and duct cells express same membrane channels and transporters, it is likely that duct cells secrete a hypertonic fluid like acinar cell. The third goal of this project is to develop a duct cell model and combine it with the acinar cell model. The combined model was used to examine the flow dependence of tear composition. The predictions agree with measurements made in anesthetized rats. en_US
dc.description.sponsorship This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. en_US
dc.format Monograph en_US
dc.format.medium Electronic Resource en_US
dc.language.iso en_US en_US
dc.publisher The Graduate School, Stony Brook University: Stony Brook, NY. en_US
dc.subject.lcsh Biology, Physiology en_US
dc.subject.other Lacrimal Gland, Mathematical Model, Water and Electrolyte Secretion en_US
dc.title Water and Ion Transport in Lacrimal Gland: Models and Experiment en_US
dc.type Dissertation en_US
dc.mimetype Application/PDF en_US
dc.contributor.committeemember Peter R. Brink en_US
dc.contributor.committeemember Chris Clausen en_US
dc.contributor.committeemember Peter Reinach en_US

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