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Involvement of surfactant proteins in ocular innate defense

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dc.contributor.advisor Ladeinde, Foluso en_US
dc.contributor.advisor Kincaid, John en_US
dc.contributor.author Lin, Xiaolan en_US
dc.contributor.other Department of Materials Science and Engineering en_US
dc.date.accessioned 2012-05-15T18:04:56Z
dc.date.accessioned 2015-04-24T14:52:43Z
dc.date.available 2012-05-15T18:04:56Z
dc.date.available 2015-04-24T14:52:43Z
dc.date.issued 2010-05-01 en_US
dc.identifier Lin_grad.sunysb_0771E_10003.pdf en_US
dc.identifier.uri http://hdl.handle.net/1951/55530 en_US
dc.identifier.uri http://hdl.handle.net/11401/72588 en_US
dc.description.abstract The ocular surface is a delicate structure that susceptible to injury and infection. Ulcerative keratitis is a painful eye condition characterized by stromal infection, ulcerative epithelium, decreased vision and cornea scar. Due to the reason that formation of vascularization, fibrosis or scarring in corneal epithelium can cause disastrous effects on vision, inflammatory response in the eye must be tightly controlled in space and time. Ocular innate defense system consists of antimicrobial peptides and immune cells that secreted or accumulated at the surface; they work in concert to eliminate intruding pathogens. Under normal circumstances, innate immunity suffices in providing quick, non-specific protection against infection. As opposed to adaptive immunity which usually takes longer and thus has more destructive effects on ocular tissues, innate immunity is more benign as its occurrence is restricted to superficial tissues such as eyelid, limbus and conjunctiva, of which the blood supplies bring immune cells to the inflamed sites for clearance of microbial and apoptotic cell debris. Surface-tension associated proteins (SP) that present in corneal epithelium and tear fluid has emerged as the new player in innate immunity of the eye. In this study, function of SP protein in corneal innate immune modulation was evaluated by using a human corneal epithelial cell line. Cells were depleted of SP by transfecting with short interfering RNA and stimulated cytokine production was determined. Conversely, exogeneous SP was found to reverse the impaired immune response in SP-depleted cells in a dose-dependent manner. This study is the first to demonstrate the role SP as immune-regulator in corneal epithelium. Combined with their known activity in direct killing of bacteria, SP may have therapeutic implications in ocular infectious diseases. en_US
dc.description.sponsorship This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. en_US
dc.format Monograph en_US
dc.format.medium Electronic Resource en_US
dc.language.iso en_US en_US
dc.publisher The Graduate School, Stony Brook University: Stony Brook, NY. en_US
dc.subject.lcsh Engineering, Materials Science -- Engineering, Biomedical en_US
dc.subject.other contact lens, cornea, inflammation, innate defense, surface tension, surfactant proteins en_US
dc.title Involvement of surfactant proteins in ocular innate defense en_US
dc.type Dissertation en_US
dc.mimetype Application/PDF en_US
dc.contributor.committeemember Gary Halada en_US
dc.contributor.committeemember Yizhi Meng en_US
dc.contributor.committeemember Richard Clark. en_US

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