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Hydrophobically modified glycol chitosan nanoparticles for targeting breast cancer microcalcification using alendronate probes

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dc.contributor.advisor Meng, Yizhi en_US
dc.contributor.author Vishnu, Kamalakannan en_US
dc.contributor.other Department of Materials Science and Engineering en_US
dc.date.accessioned 2017-09-20T16:42:33Z
dc.date.available 2017-09-20T16:42:33Z
dc.date.issued 2017-05-01 en_US
dc.identifier.uri http://hdl.handle.net/11401/76164 en_US
dc.description 93 pg. en_US
dc.description.abstract In 2016, invasive breast cancer was diagnosed in about 246,660 women and 2,600 men. An additional 61,000 new cases of in situ breast cancer was diagnosed in women. Microcalcifications are most common abnormalities detected by mammography for breast cancer, present in about 30% of all malignant breast lesions. Tumor specific biomarkers are used for targeting these abnormalities. Nanoparticles with multimodal and combinatorial therapies and conjunction of bio-ligands for specific molecular targeting using surface modifications effectually deliver a variety of drugs and are simultaneously used to image tumor progression. Alendronate, a germinal bisphosphonate conjugation as a targeting ligand would improve the nanoparticle’s direct binding to hydroxyapatite (HA) mimicking calcified spots in breast cancer lesions. In this study, the hydrophobically modified glycol chitosan (HGC) micelle was modified with alendronate surface functionalization using a biotin-avidin interaction to improve the nanomicelle’s calcification targeting ability. Biotinylated, avidinlyated hydrophobically modified iv glycol chitosan particles were linked to biotinylated alendronate via a strong biotin-avidin linkage. Cyanine 3, a red fluorescent dye was conjugated to the amine groups on HGC for visualization of micelles. The size of the nanoparticles measured was 254.0 ± 0.43 nm and 209.7 ± 1.0 nm for Cy3- BHGCA and Cy3-BHGCA-BALN nanoparticles respectively. The average surface charge was measured to be +26.9 ± 0.19 mV and +27.68 ± 0.20 mV for Cy3-BHGCA and Cy3-BHGCA- BALN nanoparticles respectively. Binding affinity using hydroxyapatite (HA) revealed that both Cy3 BHGCA BALN and Cy3 BHGCA nanoparticles displayed 95% binding in 24 hours. However, the biotin quenched nanoparticle Cy3 BHGCAB displayed 68% binding in 24 hours. The synthesis and binding chemistry was verified using Fourier transform infrared spectroscopy (FTIR). en_US
dc.description.sponsorship This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. en_US
dc.format Monograph en_US
dc.format.medium Electronic Resource en_US
dc.language.iso en_US en_US
dc.publisher The Graduate School, Stony Brook University: Stony Brook, NY. en_US
dc.subject.lcsh Materials Science en_US
dc.subject.other Alendronate, Breast cancer, HGC nanoparticles, Hydrophobically modified glycol chitosan, Microcalcification, Nanoparticle targeting en_US
dc.title Hydrophobically modified glycol chitosan nanoparticles for targeting breast cancer microcalcification using alendronate probes en_US
dc.type Thesis en_US
dc.mimetype Application/PDF en_US
dc.contributor.committeemember T, Venkatesh en_US
dc.contributor.committeemember Balázsi, Gábor. en_US

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