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The Role of Protein/Substrate Interface in the Functioning Biomedical Devices

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dc.contributor.advisor Rafailovich, Miriam H en_US
dc.contributor.author Zhang, Liudi en_US
dc.contributor.other Department of Materials Science and Engineering. en_US
dc.date.accessioned 2017-09-20T16:49:55Z
dc.date.available 2017-09-20T16:49:55Z
dc.date.issued 2015-05-01 en_US
dc.identifier.uri http://hdl.handle.net/11401/76283 en_US
dc.description 108 pg. en_US
dc.description.abstract Choosing the right materials can be critical to biomedical device performance. Usually factors such as biocompatibility, function, and cost are considered. Here we focused on the interactions between proteins, cells, and the material surfaces used for their biomedical application. The influence of surface hydrophobicity and hydrophilicity on adsorbed fibrinogen conformation, orientation, fiber formation and platelet adhesion has been investigated to understand the interactions between blood components and the materials used in blood-contacting medical devices. The results indicate that fibrinogen adsorbed to either hydrophobic or hydrophilic polymer surfaces binds platelets, implying that both surfaces are potentially thrombogenic. We present a model for surface initiation of clots, which enabled the design of a quick screen for thrombogenesis and guides the innovation and design of new anti-thrombogenic materials. In addition, the interactions of polyisoprene (PI) and commercially Gutta-percha (nanoparticles filled PI) with dental pulp stem cells (DPSC) has been evaluated. The composition of three different kinds of Gutta-percha is consistent with their mechanical properties. The biocompatibility tests show that the Gutta-percha used in our study are non-cytotxic for human adult DPSCs, and could induce biomineralization by DPSCs without the addition of a chemical inducer. Then, the study of influence of PI substrates moduli on differentiation of DPSCs show that the “hard, G>2.3MPa†substrates can improve cell proliferation and induce biomineralization without chemical inducer. The results indicate that Gutta-percha, which is a filling material currently used in endodontic practice, can be potentially used in regeneration of the tooth, rather than obduration of the canal. PI as a polymer matrix can also be used in re-engineer scaffold for tooth regeneration therapy. Hence, the study of interface between protein/cells and substrates could help the choice of materials and the design of biomedical devices. en_US
dc.description.sponsorship This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. en_US
dc.format Monograph en_US
dc.format.medium Electronic Resource en_US
dc.language.iso en_US en_US
dc.publisher The Graduate School, Stony Brook University: Stony Brook, NY. en_US
dc.subject.lcsh Materials Science en_US
dc.subject.other dental pulp stem cell, fibrinogen, platelets, polymer substrate, thrombosis, tooth regeneration en_US
dc.title The Role of Protein/Substrate Interface in the Functioning Biomedical Devices en_US
dc.type Dissertation en_US
dc.mimetype Application/PDF en_US
dc.contributor.committeemember Gersappe, Dilip en_US
dc.contributor.committeemember Jones, Keith en_US
dc.contributor.committeemember Simon, Marcia. en_US

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