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An in vitro study of the anti-biofilm properties of proanthocyanidin and chitosan in Pseudomonas syringae pv. papulans

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dc.contributor.advisor Meng, Yizhi en_US
dc.contributor.author Song, Kai en_US
dc.contributor.other Department of Materials Science and Engineering en_US
dc.date.accessioned 2017-09-20T16:50:04Z
dc.date.available 2017-09-20T16:50:04Z
dc.date.issued 2013-12-01 en_US
dc.identifier.uri http://hdl.handle.net/11401/76346 en_US
dc.description 70 pgs en_US
dc.description.abstract Biofilm-forming bacteria are a form of planktonic microorganisms that can become resistant against conventional antibiotics. Because they are difficult to eradicate, biofilm-forming bacteria are extremely problematic for the medical industry areas. Thus, materials that can distort biofilm structure would be helpful for eliminating chronic infection and decreasing bacterial resistance. The primary objective of this study is to evaluate the anti-biofilm effect of two bio-derived substances, proanthocyanidin and chitosan. Proanthocyanidins are secondary plant metabolites that are reported to have antibiotic and antioxidant functions. Chitosan is a deacetylated derivative of chitin, which is abundant in the exoskeleton of crustaceans and insects. It is reported to be a suitable substitute for conventional fungicides and can enhance the proanthocyanidin content in plants when used as an agrochemical. Chitosan-tripolyphosphate (TPP) nanoparticles, which have good neutral water solubility and are nanoscale in size, can be used as carriers for gene and drug therapy and are thus favorable to be tested as a treatment method against bacterial biofilms. In this study, the anti-biofilm and antibacterial properties of proanthocyanidin, chitosan-TPP nanoparticles and proanthocyanidins-loaded chitosan-TPP nanoparticles were tested using the model plant bacterium, Pseudomonas syringae pv. papulans (Psp), a pathogen isolated from infected apples. At a lower concentration (1 mg/mL and 2.5 mg/mL), both chitosan nanoparticles and proanthocyanidins can postpone the formation of biofilms and eventually disrupted part of the biofilm. While higher concentration (above 5 mg/mL) of chitosan nanoparticles or proanthocyanidins can eliminate most of the biofilm in this study. PAC-loaded chitosan nanoparticles also can also distort biofilms. Both proanthocyanidins and chitosan-TPP nanoparticle showed a mild antibacterial property. PAC-loaded chitosan-TPP nanoparticle exhibited a stronger and durable antibacterial property. en_US
dc.description.sponsorship This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. en_US
dc.format Monograph en_US
dc.format.medium Electronic Resource en_US
dc.language.iso en_US en_US
dc.publisher The Graduate School, Stony Brook University: Stony Brook, NY. en_US
dc.subject.lcsh Materials Science en_US
dc.subject.other antibacterial, Anti-biofilm, chitosan, chitosan/TPP nanoparticle, proanthocyanidin en_US
dc.title An in vitro study of the anti-biofilm properties of proanthocyanidin and chitosan in Pseudomonas syringae pv. papulans en_US
dc.type Thesis en_US
dc.mimetype Application/PDF en_US
dc.contributor.committeemember Gersappe, Dilip en_US
dc.contributor.committeemember Boon, Elizabeth en_US

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