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Influence of Hydrogel Substrate on Cell Growth and Virus Infection

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dc.contributor.advisor Rafailovich, Miriam en_US
dc.contributor.author Yang, Fan en_US
dc.contributor.other Department of Materials Science and Engineering en_US
dc.date.accessioned 2017-09-20T16:50:06Z
dc.date.available 2017-09-20T16:50:06Z
dc.date.issued 2013-12-01 en_US
dc.identifier.uri http://hdl.handle.net/11401/76362 en_US
dc.description 49 pgs en_US
dc.description.abstract The interaction of cells with their extracellular matrix is of great importance when cells adapt to their environment. The purpose of this thesis is to design substrates with controllable properties and to study cellular interaction on these substrates. Firstly, enzymatically cross-linked gelatin hydrogels with different elastic modulus were prepared. Then, we studied the condition of cell growth and virus infection on these hydrogels as a followed-up. In the first part of the study, we made enzymatically cross-linked gelatin hydrogels with five different elastic modulus. As a parameter of stiffness, elastic modulus varies from 2.4 KPa to 7.5 KPa based on which hydrogels are graded from soft to hard. In the second part, we studied the growth of rabbit kidney cells cultured on hydrogels of different stiffness. Growth curves of the cells were made to study the abilities of soft and hard hydrogels to support cell proliferation. Result shows that cell proliferation rate differs when using hydrogel substrates of different stiffness as substrates. In the third part of this thesis, we infected rabbit kidney cells with pseudorabies virus for a period of time . And confocal fluorescence microscopy was used to investigate the influence of hydrogels with different elastic modulus on infectivity of the virus. en_US
dc.description.sponsorship This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. en_US
dc.format Monograph en_US
dc.format.medium Electronic Resource en_US
dc.language.iso en_US en_US
dc.publisher The Graduate School, Stony Brook University: Stony Brook, NY. en_US
dc.subject.lcsh Materials Science en_US
dc.subject.other cell culture, hydrogel, infectivity en_US
dc.title Influence of Hydrogel Substrate on Cell Growth and Virus Infection en_US
dc.type Thesis en_US
dc.mimetype Application/PDF en_US
dc.contributor.committeemember Rafailovich, Miriam en_US
dc.contributor.committeemember Sokolov, Jonathan en_US
dc.contributor.committeemember Gersappe, Dilip en_US

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