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Three Novel rRNA Methyltransferases in Mammalian Mitochondria

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dc.contributor.advisor Bogenhagen, Daniel F en_US
dc.contributor.author Lee, Ken en_US
dc.contributor.other Department of Molecular and Cellular Pharmacology. en_US
dc.date.accessioned 2017-09-20T16:50:32Z
dc.date.available 2017-09-20T16:50:32Z
dc.date.issued 2014-12-01
dc.identifier.uri http://hdl.handle.net/11401/76520 en_US
dc.description 139 pg. en_US
dc.description.abstract Mammalian mitochondria maintain their double-stranded circular genome in DNA-protein complexes known as nucleoids, similar to bacteria. While bacterial and yeast mitochondrial nucleoids have been studied more extensively, much is unknown about the protein composition and events that occur at mammalian mitochondrial nucleoids. Recent work has identified many novel mitochondrial nucleoid proteins by mass spectrometry, however the functions of many of these proteins are not well understood. One of the interesting proteins identified by mass spectrometry is RNMTL1, a putative RNA methyltransferase. A search for related mitochondrial proteins in proteomic databases revealed MRM1 and MRM2 as potential RNA methyltransferases as well. The work reported in this thesis includes an initial characterization of the three novel rRNA methyltransferase proteins. We provide data on the subcellular localization of these proteins and the macromolecular complexes in which they exist. We have slightly focused more on RNMTL1, since neither RNMTL1 nor any known homologues have previously been characterized, and provide data on its protein interacting partners. We also provide evidence that RNMTL1 has an important role in mammalian mitochondrial translation. In addition, we have assigned the three methyltransferases to their specific substrate sites on the mitochondrial large ribosomal subunit rRNA. MRM1, MRM2 and RNMTL1 methylate the 2'-O-ribose of G1145, U1369 and G1370, respectively. These methyltransferases are also likely involved in mitochondrial ribosome assembly and/or stability. en_US
dc.description.sponsorship This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. en_US
dc.format Monograph en_US
dc.format.medium Electronic Resource en_US
dc.language.iso en_US en_US
dc.publisher The Graduate School, Stony Brook University: Stony Brook, NY. en_US
dc.subject.lcsh Pharmacology en_US
dc.subject.other Mammalian, Mitochondria, Ribosome, rRNA methylation en_US
dc.title Three Novel rRNA Methyltransferases in Mammalian Mitochondria en_US
dc.type Dissertation en_US
dc.mimetype Application/PDF en_US
dc.contributor.committeemember Demple, Bruce en_US
dc.contributor.committeemember Garcia-Diaz, Miguel en_US
dc.contributor.committeemember Karzai, Wali en_US
dc.contributor.committeemember Czaplinski, Kevin. en_US

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