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A Targeted CRISPR-Cas9 Screen in AML Cells

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dc.contributor.advisor Dean, Neta en_US
dc.contributor.author Chiappone, Sam Blake en_US
dc.contributor.other Department of Biochemistry and Cell Biology en_US
dc.date.accessioned 2017-09-20T16:51:25Z
dc.date.available 2017-09-20T16:51:25Z
dc.date.issued 2015-12-01 en_US
dc.identifier.uri http://hdl.handle.net/11401/76906 en_US
dc.description 43 pgs en_US
dc.description.abstract The genetic underpinnings of a cancer are key to understanding the etiology of the disease and thus to providing a cure, because any robust cure of the disease will need to target a therapeutic window, a gene or a group of genes that are different between the cancerous cells and the normal cells. The research shown in this thesis gives insight into the genetic causes of acute myelogenous leukemia in an attempt to find a therapeutic window for this disease. A genetic screen using CRISPR-Cas9 is designed and conducted in a cell line designated RN 2-5 that was determined to recapitulate the acute myelogenous leukemia phenotype in culture and in a mouse model of the disease. Following up on the most important hits of this screen will very likely lead to important therapeutic targets. Two bioinformatics methods, a machine-learning algorithm and a statistical approach, are also used to identify key features of the expression of genes targeted in the screen. A combination of genetic screening and bioinformatics will have great efficacy in the future in finding genes differentially expressed, and differentially required, by cancer cells versus normal cells. en_US
dc.description.sponsorship This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. en_US
dc.format Monograph en_US
dc.format.medium Electronic Resource en_US
dc.language.iso en_US en_US
dc.publisher The Graduate School, Stony Brook University: Stony Brook, NY. en_US
dc.subject.lcsh Biochemistry en_US
dc.title A Targeted CRISPR-Cas9 Screen in AML Cells en_US
dc.type Thesis en_US
dc.mimetype Application/PDF en_US
dc.contributor.committeemember Zhang, Lingbo en_US
dc.contributor.committeemember Stenlund, Arne en_US

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