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Role of Runt, Even-skipped, and Odd-paired in Regulating Enhancer activity in the Drosophila embryo

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dc.contributor.advisor Gergen, John P en_US
dc.contributor.author Higgins, Michael Luis en_US
dc.contributor.other Department of Biochemistry and Structural Biology en_US
dc.date.accessioned 2017-09-20T16:51:29Z
dc.date.available 2017-09-20T16:51:29Z
dc.date.issued 2016-12-01
dc.identifier.uri http://hdl.handle.net/11401/76941 en_US
dc.description 106 pg. en_US
dc.description.abstract The Drosophila runt gene is the founding member of the RUNX transcription factor family, a group of conserved genes that have vital roles in multiple developmental pathways throughout the animal kingdom. Runt plays important roles in segmentation, as well as in neurogenesis and sex determination in the Drosophila embryo. As a transcription factor, Runt is both an activator and a repressor of its target genes depending on the context. Sloppy-paired (slp1) is a target of runt’s activity as a pair-rule transcription factor in the segmentation pathway that is both activated and repressed by Runt. Two cis-regulatory enhancers of slp1, a Distal Early Stripe Element (DESE) and a Proximal Early Stripe Element (PESE), that mediate regulation by Runt and other pair-rule transcription factors have been characterized. The transcription factor, encoded by the odd-paired (Opa) gene, Opa is an important activator for both DESE and PESE. This thesis describes progress towards identifying Opa binding sites in these enhancers. The PESE enhancer is repressed in cells that co-express Runt and the transcription factor encoded by the fushi-tarazu (Ftz) gene. This thesis investigates the roles of Ftz and the Runt binding sites in PESE in this repression. Prior work on the regulation of the DESE and PESE enhancers revealed a non-additive interaction that could be explained if repression that involves preventing release of promoter-proximal paused RNA Polymerase II dominantly interferes with the ability of other enhancers to drive expression from this same promoter. This proposal is investigated by examining the effects of the slp1 enhancers on expression driven by a heterologous enhancer from the short-gastrulation (sog) gene. The results of these experiments and their implications on our understanding of transcription regulation during animal development are discussed. en_US
dc.description.sponsorship This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. en_US
dc.format Monograph en_US
dc.format.medium Electronic Resource en_US
dc.language.iso en_US en_US
dc.publisher The Graduate School, Stony Brook University: Stony Brook, NY. en_US
dc.subject.lcsh Developmental biology -- Genetics -- Biochemistry en_US
dc.subject.other enhancers, even-skipped, oddpaired, runt en_US
dc.title Role of Runt, Even-skipped, and Odd-paired in Regulating Enhancer activity in the Drosophila embryo en_US
dc.type Dissertation en_US
dc.mimetype Application/PDF en_US
dc.contributor.committeemember Hollingsworth, Nancy M en_US
dc.contributor.committeemember Martin, Benjamin L en_US
dc.contributor.committeemember Luk, Ed en_US
dc.contributor.committeemember Small, Stephen J. en_US


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