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Exploring RNA Binding Pattern of Rim4 in Meiosis

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dc.contributor.advisor Futcher, Bruce en_US
dc.contributor.advisor Neiman, Aaron en_US
dc.contributor.author Xiao, Che en_US
dc.contributor.other Department of Genetics en_US
dc.date.accessioned 2017-09-20T16:53:07Z
dc.date.available 2017-09-20T16:53:07Z
dc.date.issued 2015-08-01 en_US
dc.identifier.uri http://hdl.handle.net/11401/77636 en_US
dc.description 50 pgs en_US
dc.description.abstract In <italic>Saccharomyces cerevisiae</italic>, translation is regulated in meiosis <super>1</super>. Rim4 is an RNA binding protein with two RNA recognition motifs (RRMs). Previous studies <super>2</super> have shown that point mutations in either RRM knock out the translational repression of <italic>CLB3</italic>, <italic>SPO20</italic>, <italic>GIP1</italic> and <italic>SPS1</italic> in meiosis I, suggesting that the translational control of these genes may be due to Rim4. We wished to see the full range of RNAs bound by Rim4. We applied RIP-chip (RNA Immunoprecipitation/microarray) and CLIP-seq (<italic>in vivo</italic> Crosslinking and Immunoprecipitation/sequencing) to identify the RNAs co-immunoprecipitated with Rim4 in a synchronized meiosis I culture. The translationally-repressed genes co-clustering with <italic>CLB3</italic>, and translationally-repressed genes co-clustering with <italic>AMA1</italic> in ribosome footprint data <super>1</super> were enriched in Rim4 immunoprecipitates compared to the negative controls. Therefore, these two groups of translationally repressed genes seem to be the targets of Rim4. Some other genes without translational repression in meiosis I were also enriched in Rim4 immunoprecipitates. en_US
dc.description.sponsorship This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. en_US
dc.format Monograph en_US
dc.format.medium Electronic Resource en_US
dc.language.iso en_US en_US
dc.publisher The Graduate School, Stony Brook University: Stony Brook, NY. en_US
dc.subject.lcsh Biochemistry en_US
dc.subject.other budding yeast, meiosis, Rim4, RNA binding protein, translational regulation en_US
dc.title Exploring RNA Binding Pattern of Rim4 in Meiosis en_US
dc.type Thesis en_US
dc.mimetype Application/PDF en_US

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